Lactobacillus Ferment, Water Soluble Preservative

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Lactobacillus is a genus of bacteria that is heralded for its use as a probiotic, beneficial for human digestion. Itis traditionally used as a natural fermenting agent for use in the production of yogurt, kimchi, sauerkraut, kefir and a variety of other fermented foods.

Lactobacillus Ferment acts as a natural broad-spectrum antimicrobial in aqueous products and in oil-in-water emulsions by acidifying the environment and producing antimicrobial peptides called bacteriocins. Additionally, it lends nutritive skin conditioning benefits to personal care formulations.

As the media continues to focus its attention on the risks of parabens and other synthetic preservatives, product formulators and manufacturers are facing the growing challenge of procuring natural alternatives that provide suitable broad spectrum protection.

Natural Sourcing is pleased to offer manufacturers and formulators a range of premium quality natural preservatives, including Lactobacillus Ferment.


  • Product Form: Liquid
  • Solubility: Water
  • Color: Clear, Slightly Hazy
  • Odor: Very Faint, Characteristic Aroma
  • Viscosity: Thin
  • pH: 3.0-7.0
  • Skin Conditioning
  • Antimicrobial (Antibacterial and Antifungal)
  • Microorganisms Tested:
    • E. coli
    • P. aeruginosa
    • S. aureaus
    • C. albicans
    • A. brasiliensis


Lactobacillus Ferment is well suited for use as a natural antimicrobial in the production of skin care, hair care and cosmetic formulations. In addition to its application as a natural antimicrobial, Lactobacillus Ferment also helps to condition the skin.

Lactobacillus Ferment is water soluble and can be added to water based formulations or during the water phase of emulsions that maintain a pH range of 3-8. It is recommended that Lactobacillus Ferment be added during the cooling phase at temperatures below 158 degrees F (70 degrees C) and added prior to the addition of thickeners such as xanthan gum or Carbopol Ultrez 10.

Recommended Usage Rate: 2 - 4%

All formulations should be challenge tested by a qualified laboratory to ensure that each batch is properly preserved. The method of production, the specific components of the formulation, the environment in which the batch is formulated and the method of packaging all play a crucial role in the resulting shelf life of a batch. Therefore, we cannot guarantee your results when incorporating natural preservatives into your formulations. Only lab challenge testing can determine how and if a preservative is achieving the desired results.

Storage Information:

Mix well before use. Store in a cool, dry location. The ideal storage temperature range is 73-78 degrees Fahrenheit (23-25 degrees Celsius). Do not freeze.

Test Results

The ability of Lactobacillus Ferment to inhibit the growth of a variety of bacteria and fungi was determined using the Minimum Inhibitory Concentration (MIC) test. The results are illustrated below, showing that this material provides broad spectrum antimicrobial protection.

E. coli P. aeruginosa S. aureus A. brasiliensis C. albicans
MIC% 0.75 1.00 1.00 1.00 0.75
Table 1: MIC data for Lactobacillus Ferment.

A Double Challenge Test using 2% Lactobacillus Ferment was also conducted to evaluate the ability of the product to provide antimicrobial protection in finished formulas. A basic O/W emulsion was used as the base. The samples were inoculated with E. coli, P. aeruginosa, S. aureus, A. brasiliensis and C. albicans and incubated for 28 days. During this period, samples were periodically collected and tested for the presence of viable microorganisms. Following the initial 28 days of incubation, the samples were re-inoculated with the microbial cultures for another period of 28 days. The results are shown below.

E. coli P. aeruginosa S. aureus A. brasiliensis C. albicans
Inoculum (Initial) 7.0x106 7.0x105 5.1x106 1.7x106 4.7x106
Day 0 >99.999% 99.857% >99.999% 99.996% 99.997%
Day 7 >99.999% >99.999% >99.999% >99.999% >99.999%
Day 14 >99.999% >99.999% >99.999% >99.999% >99.999%
Day 21 >99.999% >99.999% >99.999% >99.999% >99.999%
Day 28 >99.999% >99.999% >99.999% >99.999% >99.999%
Inoculum (Reinoculated) 8.2x107 1.6x106 1.0x106 3.3x106 1.7x106
Day 7 >99.999% 99.985% 99.987% 99.977% 99.975%
Day 14 >99.999% >99.999% >99.999% >99.999% >99.999%
Day 21 >99.999% >99.999% >99.999% >99.999% >99.999%
Day 28 >99.999% >99.999% >99.999% >99.999% >99.999%
Table 2: Challenge Test results for 2% Lactobacillus Ferment in O/W emulsion.

Origin: USA
INCI: Lactobacillus Ferment
Extraction Method: Controlled Fermentation, Lysozyme Added to Facilitate Controlled Cell Lysis, Then Filtered
Shelf Life: 1 Year

Documents: Safety Data Sheet   Specifications

Wholesale Pricing: [wholesale pricing info]
  1 fl oz., $10.38
  2 fl oz., $17.80
  4 fl oz., $32.63
  8 fl oz., $59.32
  16 fl oz., $106.78
  32 fl oz., $189.82
  64 fl oz., $332.19
  1 gal., $616.93 * Temporarily Out of Stock
  2 gal., $1,138.94 * ^ Temporarily Out of Stock
  5 gal., $2,372.80 * ^ Temporarily Out of Stock


*This item does not qualify for any promotional discounts.

^This product will ship separately.

Customer Ratings and Reviews

Average Rating:   

Rating: 5 out of 5

Reviewed By: Susan

Posted: Tuesday, January 03, 2017

Location: Middleville

Comments: Great price. Will purchase again.

Rating: 5 out of 5

Reviewed By: Erin

Posted: Wednesday, December 21, 2016

Location: Madison, WI

Comments: I purchased Lactobacillus Ferment to add as a preservative. My first batch was made on 12/11/16 and so far so good. Thank you for a wonderful product and website with all the information I needed about your products.

Rating: 5 out of 5

Reviewed By: Mindy

Posted: Thursday, July 21, 2016

Location: Cocoa, FL

Comments: This product works great in many products that I want to preserve naturally. It does work better combined with another preservative that is used for preventing mold and yeast. It makes your skin very soft. I like adding it to face toners, cleansers and lotions.

Total Number of Ratings: 3
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